FEDER - ANSES - AVEq-ACTIVE - TREMPLIN
Kohesio
AEP AGENCE NATIONALE SECURITE SANITAIRE ALIMENTATION TRAVAIL
The general objective of this study is to characterize and determine the mechanisms of action of molecules which have shown an antiviral effect against two equine respiratory viruses (AVE and HVE-1), following a screening of 2,900 molecules, in order to develop effective strategies for combating and controlling these diseases responsible for major economic losses for the equine industry. In this context, we have five objectives: Objective 1: Characterize the invitro pharmacological properties of molecules for which “hits” were obtained following high-throughput screening of 2,900 molecules from different public chemical libraries. Objective 2: Evaluate the effect of these molecules on gene expression of infected and non-infected equine cells. Objective 3: Evaluate the effect of these molecules on the activity of cellular kinases in our equine cell model. Objective 4: Evaluate the metabolic stability of molecules active against AVE and HVE-1 in an in vitro equine biological model (horse liver microsomes). Objective 5: Study the pharmacokinetics and toxicity of these molecules in horses. The originality of our project is based on the search for molecules with antiviral properties against two equine respiratory viruses. Indeed, to date no molecule has marketing authorization (AMM) to fight against viral infections in equines according to the National Veterinary Medicines Agency (ANMV). This project will therefore make it possible to expand the range of treatments available to combat equine viruses and the consequences of these infections on the equine industry. To do this, the project will be structured around four “work packages” WP1: Characterize the antiviral power of molecules/compounds from different chemical libraries WP2: Evaluate the effect of molecules on cells WP3: Pharmacokinetic and toxicological studies. WP0: Coordination and communication of project results.
Smarter Europe / Research and innovation / Research and innovation activities in public research centres and centres of competence including networking